Pestene powder is one of the most recognised treatments for lice and mites in poultry. Treatment areas to focus application on poultry are specifically the vent area (fluffy bottom under the tail), under each wing and around the neck and breast. Much easier if two people do this, one to hold the chicken still and move him/her around for powder application and the other to do the sprinkling and ruffling of the feathers to ensure that the powder sits on the skin. When released, the chicken will give an undignified shake to resettle her feathers but this is normal. Repeat applications might be needed one week after the first for heavy infestations. Pestene is also suitable for sprinkling around nesting areas and around the coop for added protection.